ENVÍO GRATIS - PAGO SEGURO - GARANTÍA DE DEVOLUCIÓN DE DINERO

¿Te gusta este producto? Si es así, compártelo con tus amigos.

Descripción

Membrane proteins fulfil a wide variety of essential functions in the cell and many are (potential) drug targets. Since their natural abundance is usually very low, most membrane proteins have to be over-expressed for functional and structural studies. Unfortunately, over-expression of membrane proteins in E. coli is usually toxic. I have studied the effects of the expression of membrane proteins, including the human KDEL receptor, on BL21(DE3) and its derivatives. Saturation of the Sec translocon, a cytoplasmic membrane associated protein conducting channel that mediates the insertion/ biogenesis of membrane proteins, appeared to be the prime reason for the toxicity of membrane protein over-expression. The obtained data have resulted in many leads and ideas to further improve membrane protein over-expression yields in E. coli, e.g. we have engineered a plasmid (pLemo) that helps avoiding saturation of the Sec translocon upon membrane protein over-expression in E. coli.

Contribuyente

Autor Mirjam Klepsch

Información

DUIN 1P976KF8EGU

GTIN 9783848419692

Idioma Inglés

Número de páginas 88

Product type Libro de bolsillo

Into the Membrane and Beyond

Improving Membrane Protein Over-Expression in Escherichia coli

Mirjam Klepsch

47,47 €

Vendedor: Dodax EU

Fecha de entrega: entre viernes, 21. agosto y martes, 25. agosto

Condición: Nuevo

IVA incluído - Envío GRATIS
1
Agregar a la cesta Agregar a la cesta
47,47 €
IVA incluído - Envío GRATIS