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Fungal Secondary Metabolism - Methods and Protocols

por Humana Press
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116,48 €
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Humana Press Fungal Secondary Metabolism - Methods and Protocols
Humana Press - Fungal Secondary Metabolism - Methods and Protocols

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Descripción

Filamentous fungi have long been known for their ability to produce an enormous range of unusual chemical compounds known as secondary metabolites, many of which have potentially useful antibiotic or pharmacological properties. Recent focus on fungal genomics coupled with advances in detection and molecular manipulation techniques has galvanized a revitalization of this field.  Fungal Secondary Metabolism: Methods and Protocols is aimed at providing the key methodologies currently in use and necessary for accessing and exploiting the natural product information provided by the genomes of this large and varied kingdom. Written by active researchers in the field, the chapters deal with all the steps necessary, from optimization of fungal culture conditions for metabolite production, through rapid genome sequencing and bioinformatics, and genetic manipulations for functional analysis, to detection and testing of metabolites. In addition, chapters on basic science address approaches to the genetic regulation, protein biochemistry, and cellular localization of the biosynthetic pathways. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.


 

Practical and hands-on, Fungal Secondary Metabolism: Methods and Protocols encourages new investigators to enter the field and expands upon the expertise and range of skills of those already researching fungal natural products.

Detalles de producto

Ilustraciones:
11 Tabellen
Indice:

1. Library Preparation and Data Analysis Packages for Rapid Genome Sequencing


            Kyle R. Pomraning, Kristina M. Smith, Erin L. Bredeweg, Lanelle R. Connolly, Pallavi A. Phatale, and Michael Freitag


2. Bioinformatics Approaches and Software for Detection of Secondary Metabolic Gene Clusters


            Natalie D. Fedorova, Venkatesh Moktali, and Marnix H. Medema


3. Media and Growth Conditions for Induction of Secondary Metabolite Production


            Jens C. Frisvad


4. Manipulating Filamentous Fungus Chemical Phenotypes by Growth on Nutritional Arrays


            José R. Tormo, Francisco J. Asensio, and Gerald F. Bills


5. The “FERMEX” Method for Metabolite-Enriched Fungal Extracts


            Gerald F. Bills, Anne W. Dombrowski, and Michael A. Goetz


6. The Chemical Identification and Analysis of Aspergillus nidulans Secondary Metabolites


            James F. Sanchez and Clay C.C. Wang


7. Detection and Purification of Non-Ribosomal Peptide Synthetase Products in Neosartorya fischeri


            Beate Wollinsky and Shu-Ming Li


8. Production, Detection, and Purification of Clavine-Type Ergot Alkaloids


            Christiane Wallwey and Shu-Ming Li


9. Analysis of Volatile Compounds Emitted by Filamentous Fungi Using Solid Phase Microextraction - Gas Chromatography/Mass Spectrometry (SPME-GC/MS)


            Ludmila V. Roze, Randolph M. Beaudry, and John E. Linz


10. Tools for Manipulation of Secondary Metabolism Pathways: Rapid Promoter Replacements and Gene Deletions in Aspergillus nidulans


            C. Elizabeth Oakley, Heather Edgerton-Morgan, and Berl R. Oakley


11. Fast and Easy Method for Construction of Plasmid Vectors Using Modified Quick-Change Mutagenesis


            Jin Woo Bok and Nancy P. Keller


12. Reconstitution of a Secondary Metabolite Biosynthetic Pathway in a Heterologous Fungal Host


            Takayuki Itoh, Tetsuo Kushiro, and Isao Fujii


13. Multi-Copy Genetic Screen in Aspergillus nidulans


            Hee Soo Park and Jae-Hyuk Yu


14. Identification of Protein Complexes from Filamentous Fungi with Tandem Affinity Purification


            Özgür Bayram, Özlem Sarikaya Bayram, Oliver Valerius, Bastian Jöhnk, and Gerhard H. Braus


15. Correlating Secondary Metabolite Production with Genetic Changes Using Differential Analysis of 2D NMR Spectra


            Ry R. Forseth and Frank C. Schroeder


16. Chromatin Immunoprecipitation (ChIP) Analysis in Filamentous Fungi


            Yazmid Reyes-Dominguez, Stefan Boedi, and Joseph Strauss


17. Targeted Proteomics for Metabolic Pathway Optimization


            Tanveer S. Batth, Jay D. Keasling, and Christopher J. Petzold


18. Investigation of In Vivo Protein Interactions in Aspergillus Spores


            Kwang Cheol Jeong and Jae-Hyuk Yu


19. Purification of a Vesicle-Vacuole (V) Fraction from Aspergillus


            Anindya Chanda, Ludmila V. Roze, and John E. Linz


20. Use of the Hollow Fiber Assay for the Discovery of Novel Anticancer Agents from Fungi


            Cedric J. Pearce, Daniel D. Lantvit, Qi Shen, David Jarjoura, Xiaoli Zhang, Nicholas H. Oberlies, David J. Kroll, Mansukh C. Wani, Jimmy Orjala, Djaja D. Soejarto, Norman R. Farnsworth, Esperanza J. Carcache de Blanco, James R. Fuchs, A. Douglas Kinghorn, and Steven M. Swanson


21. Molecular Analysis of Fungal Gene Expression upon Interkingdom Competition with Insects


            Ulrike Fohgrub and Frank Kempken


            José R. Tormo, Francisco J. Asensio, and Gerald F. Bills


Editor:
Keller, Nancy P.;Keller
Turner, Geoffrey;Turner
Observaciones:
Presents methods covering fungal cultivation and transformation to metabolite isolation, purification, and analysis


Features detailed and practical laboratory protocols


Includes expert tips on troubleshooting and avoiding known pitfalls


Includes supplementary material: sn.pub/extras


Includes supplementary material: sn.pub/extras

Tipo de medio:
Tapa blanda
Editorial:
Humana Press
Revisado:

From the book reviews:

"The book 'Fungal Secondary Metabolism: Methods and protocols' present methodologies currently used for accessing and discovering the information on natural products in the genome of filamentous fungi. ... Should be available in all college, research laboratories and university libraries since this information will be very useful for researchers who study fungal secondary metabolites. In addition, this information will be helpful for any researcher working on the fungal genomics." (K. W. Thilini Chethana and Kevin D. Hyde, Fungal Diversity, December, 2014)

"This is very much a how-to-do book, prepared by two particularly distinguished mycologists, and designed for those wishing to investigate the chemical possibilities of filamentous fungi. ... Steps to be commended are detailed and practical procedures illustrated, accompanied by discussions of the strength and weaknesses of different packages. ... The editors and publishers must be congratulated in the extent to which they have managed to marshal their contributors into such a common and lucid style." (IMA Fungus, Vol. 3 (2), 2012)

Idioma:
Inglés
Edición:
Softcover reprint of the original 1st ed. 2012
Número de páginas:
288
Resumen:
This Methods in Molecular Biology™ volume provides key methodologies for accessing and exploiting natural product information provided by the genomes of filamentous fungi. Includes materials and reagents lists, step-by-step protocols and troubleshooting tips.

Datos base

Tipo de producto:
Libro de bolsillo
Fecha de aparición:
23 de agosto de 2016
Dimensiones del paquete:
0.252 x 0.176 x 0.018 m; 0.34 kg
GTIN:
09781493959600
DUIN:
3LEL6KE53P0
116,48 €
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