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AMPK - Methods and Protocols

por Springer US
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142,44 €
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Springer US AMPK - Methods and Protocols
Springer US - AMPK - Methods and Protocols

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Entrega: entre viernes, 27 de mayo de 2022 y martes, 31 de mayo de 2022
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Descripción

This volume explores the latest methods used to study AMPK by computational, biochemical, biophysical, cellular, and ex vivo and in vivo approaches. The chapters in this book cover specific topics, such as methods to measure change in cellular energy metabolism and analyze metabolic pathways regulated by AMPK; bioinformatics tools to identify AMPK targets; knockdown of AMPK by CRISPR-Cas9; production and crystallization of full-length human AMP-activated protein kinase; cell-free assays to measure the effects of regulatory ligands on AMPK; use of sensors of AMPK activity; AMPK protein interaction by yeast two-hybrid; the role of AMPK in inflammation and autophagy; analyzing the AMPK function in C. elegans and mammals (with special focus on skeletal muscle, blood vessels, kidneys, pancreatic islets and hypothalamus); and human γ2 AMPK mutations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting edge and thorough, AMPK: Methods and Protocols is a valuable resource for graduate students, postdoctoral researchers, and established investigators who are interested in the richly diverse AMPK field.

Detalles de producto

Ilustraciones:
42 SW-Abb., 72 Farbabb.
Indice:

Preface…


Table of Contents…


Contributing Authors…


1. Production and Crystallization of Full-Length Human AMP-Activated Protein Kinase (a1b1y1)


Julia A. Hubbard, Bing Xiao, and Jon R. Wilson


2. Visualizing AMPK Drug Binding Sites through Crystallization of Full Length Phosphorylated a2b1y1 Heterotrimer


Christopher G. Langendorf, Jonathan S. Oakhill, and Bruce E. Kemp


3. Biophysical Interactions of Direct AMPK Activators


Ravi G. Kurumbail, Graham M. West, Venkatasubramanian Dharmarajan, Kris A. Borzilleri, Jane M. Withka, Jessica Ward, Allan R. Reyes, Francis Rajamohan, Patrick R. Griffin, and Matthew F. Calabrese


4. Biochemical Measurement of Glycogen: Method to Investigate the AMPK-Glycogen Relationship


Elite Possik and Arnim Pause


5. Cell-Free Assays to Measure Effects of Regulatory Ligands on AMPK


Fiona A. Fyffe, Simon A. Hawley, Alexander Gray, and D. Grahame Hardie


6. Applications of NMR and ITC for the Study of the Kinetics of Carbohydrate Binding by AMPK β-Subunit Carbohydrate Binding Modules


Paul R. Gooley, Ann Koay, and Jesse I. Mobbs


7. Bioinformatics Approach to Identify Novel AMPK Targets


Brendan Gongol, Traci Marin, David A. Johnson, and John Y-J. Shyy


8. Studying AMPK in an Evolutionary Context


Arpit Jain, Valentin Roustan, Wolfram Weckwerth, and Ingo Ebersberger


9. AMPK Protein Interaction Analyses by Yeast Two-Hybrid


Pascual Sanz, Rosa Viana, and Maria Adelaida Garcia-Gimeno


10. Transient Expression of AMPK Heterotrimer Complexes in Mammalian Cells


Jonathan S. Oakhill, John W. Scott, and Toby A. Dite


11. Knockdown of Human AMPK using the CRISPR-Cas9 Genome-Editing System


Adrien Grenier, Pierre Sujibert, Séverine Olivier, Hélène Guermouche, Johanna Mondésir, Olivier Kosmider, Benoit Viollet, and Jérôme Tamburini


12. Compound C/Dorsomorphin – Its Use and Misuse as an AMPK Inhibitor


Biplab Dasgupta and William Seibel


13. Identifying the Heterotrimeric Complex Stoichiometry of AMPK in Skeletal Muscle by Immunoprecipitation


Jesper B. Birk and Jørgen F. P. Wojtaszewski


14. Kinase Activity Determination of Specific AMPK Complexes/Heterotrimers in Skeletal Muscle


Jesper B. Birk and Jørgen F. P. Wojtaszewski


15. Determination of Adenine Nucleotide Concentrations in Cells and Tissues by High Performance Liquid Chromatography


Noemí García-Tardón and Bruno Guigas


16. Intact Cell Assays to Monitor AMPK and Determine the Contribution of the AMP-Binding or ADaM Sites to Activation


Simon A. Hawley, Fiona A. Fyffe, Fiona M. Russell, Graeme J. Gowans, and D. Grahame Hardie


17. Cellular Application of Genetically-Encoded Sensors and Impeders of AMPK


Takafumi Miyamoto, Elmer Rho, Allen Kim, and Takanari Inoue


18. Assessing Mitochondrial Bioenergetics by Respirometry in Cells or Isolated Organelles


Guillaume Vial and Bruno Guigas


19. Study of AMPK Regulated Metabolic Fluxes in Neurons using the Seahorse XFe Analyzer


Claudia Marinangeli, Jérome Kluza, Philippe Marchetti, Luc Buée, and Valérie Vingtdeux


20. Investigating the Role of AMPK in Inflammation


Sarah J. Mancini and Ian P. Salt

21. Studying the Role of AMPK in Cardiac Hypertrophy and Protein Synthesis


Florence Mailleux, Christophe Beauloye, Jean-Luc Balligand, Sandrine Horman, and Luc Bertrand


22. Assessment of AMPK-Stimulated Cellular Long-Chain Fatty Acid and Glucose Uptake


Joost J.F.P. Luiken, Dietbert Neumann, Jan F.C. Glatz, Will A. Coumans, Dipanjan Chanda, and Miranda Nabben

23. Measurement of AMPK-Induced Inhibition of Lipid Synthesis Flux in Cultured Cells


Marc Foretz and Benoit Viollet


24. Studying the Role of AMPK in Autophagy


Sarah Krieg, Bernhard Lüscher, Jörg Vervoorts, and Marc Dohmen


25. Determining AMPK Activation via the Lysosomal v-ATPase-Ragulator-AXIN/LKB1 Axis


Chen-Song Zhang, Mengqi Li, Yue Zong, and Sheng-Cai Lin


26. Manipulation and Measurement of AMPK Activity in Pancreatic Islets


Aida Martinez-Sanchez, Marie-Sophie Nguyen-Tu, Isabelle Leclerc, and Guy A. Rutter


27. Analyzing AMPK Function in the Hypothalamus


Patricia Seoane-Collazo and Miguel López


28. Using Ex Vivo Kidney Slices to Study AMPK Effects on Kidney Proteins


Renee Rao, Kazuhiro Omi, Roshan Rajani, Hui Li, and Nuria M. Pastor-Soler


29. A Flow Cytometry-Based Protocol to Measure Lymphocyte Viability upon Metabolic Stress


Sébastien Denanglaire, Tiphène Pirnay, Oberdan Leo, and Fabienne Andris


30. Methods to Evaluate AMPK Regulation of Macrophage Cholesterol Homeostasis


Nicholas D. LeBlond and Morgan D. Fullerton


31. Modulation of Vascular Function by AMPK: Assessment of NO Bioavailability and Surrogates of Oxidative Stress


Swenja Kröller-Schön, Andreas Daiber, and Eberhard Schulz



32. Measurement of Reactive Oxygen Species (ROS) and Mitochondrial ROS in AMPK Knockout Mice Blood Vessels


Qilong Wang and Ming-Hui Zou


33. Studying the Role of AMPK in Angiogenesis


Katrin Spengler, Silke Lindenmüller, Nderim Kryeziu, Anne Knierim, and Regine Heller


34. Analysis of Muscle Stem Cell Fate through Modulation of AMPK Activity


Marine Theret, Linda Gsaier, Sabrina Ben Larbi, Michèle Weiss-Gayet, and Rémi Mounier


35. Evaluating the Role of Host AMPK in Leishmania Burden


Diana Moreira, Jérôme Estaquier, Anabela Cordeiro-da-Silva, and Ricardo Silvestre


36. Analysis of Transgenerational Phenotypes Following Acute Atarvation in AMPK-Deficient C. elegans


Emilie Demoinet and Richard Roy


37. Human γ2 AMPK Mutations


Arash Yavari, Dhruv Sarma, and Eduardo B. Sternick 

Editor:
Neumann, Dietbert;Neumann
Viollet, Benoit;Viollet
Observaciones:
Includes cutting-edge methods and protocols


Provides step-by-step detail essential for reproducible results


Contains key notes and implementation advice from the experts

Tipo de medio:
Tapa blanda
Editorial:
Springer US
Idioma:
Inglés
Edición:
Softcover reprint of the original 1st ed. 2018
Número de páginas:
626

Datos base

Tipo de producto:
Libro de bolsillo
Dimensiones del paquete:
0.254 x 0.178 x 0.034 m;
GTIN:
09781493992492
DUIN:
DL74PLUEDQB
142,44 €
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